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1. Ultrasonic Pregnancy Tester: For sows 25 to 30 days after mating, the correct use of pregnancy tester identification, the accuracy rate of 98.5%. (1) Check the pregnancy instrument is working properly. Press the [ON" button on the front center of the pregnancy instrument with your thumb to hear a tone, which means that the instrument is working properly. (2) Test time. The earliest test can be conducted within 18 days of mating, and no later than 75 days after sow mating. Usually measured 30 days after mating, as long as the correct use of pregnancy test method, the accuracy rate of pregnancy diagnosis of more than 98.5%. (3) Test methods. Before testing, smear the probe and measurement site of the pregnancy instrument with paraffin oil (or vegetable oil, engine oil) so that the probe is in good contact with the skin to ensure the accuracy of the measurement. When testing, it is best to calm the sow (tested during feeding) and place the probe on the right side of the sow to be diagnosed, 3 cm on the base line of the nipple, and 5 cm at the front edge of the sow of the sow. Align the sow's body to scan the uterus, scan the front 45 degrees, and scan 45 degrees to the side (aligning the left front shoulder). When the pregnancy instrument emits a continuous "tap--" tone, it means that the sow has become pregnant; when the intermittent "sputum, fleas, fleas, and fleas" is issued, it means that the sow is not pregnant. 2. Observing method: Let a boar walk around in front of the sows in 18 to 24 days after mating, and it is a good way for the estrus to check the behavior of the sow. Sows no longer have estrus at about 21 days after mating, and can initially be judged to be pregnant. When the second estrus cycle is still not in estrus, the sow has been conceived. Behavioral characteristics: Quiet, snooty, easy to lick, abdominal circumference gradually increased; nipple began to darken, gently elongated nipples, nipples at the base of the nipple appear black purple; from the rear of the sow to observe the arrangement of nipples between the two hind legs It can be seen that the front of the nipple opens outwards, and the base of the nipple expands and rises. The performance of individual piglets is also not necessarily pregnancy, as noted above. In addition, the pregnant sow is shiny and shiny, and her eyes are bright and shiny, and the joints under the vulva contract upwards into a line.

This kit can only be used for scientific research, not for medical diagnosis Instruction manual of human anti-HCG antibody (HCG-Ab) ELISA detection kit Detection principle The kit uses double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with the capture antibody of human anti-HCG antibody (HCG-Ab), add the specimen, standard, and HRP-labeled detection antibody in sequence, incubate and wash thoroughly. The color is developed with the substrate TMB, which is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The color depth is positively correlated with the human anti-HCG antibody (HCG-Ab) in the sample. Measure the absorbance (OD value) at 450nm with a microplate reader to calculate the sample concentration. Sample collection, processing and storage methods 1. Serum: Use a test tube that does not contain pyrogens and endotoxins. Avoid any cell stimulation during the operation. After collecting blood, centrifuge at 3000 rpm for 10 minutes to quickly and carefully separate serum and red blood cells. 2. Plasma: EDTA, citrate or heparin anticoagulation. Take the supernatant by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: centrifuge at 3000 rpm for 10 minutes to remove particles and polymers. 4. Tissue homogenate: Add tissue to the right amount of saline and mash. Take the supernatant by centrifugation at 3000 rpm for 10 minutes. 5. Preservation: If the sample is not tested in time after collection, please aliquot it in one dose and freeze it at -20 ℃ to avoid repeated freezing and thawing. Thaw at room temperature and ensure that the sample is thawed evenly and fully. Bring your own items 1. Microplate reader (450nm) 2. High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL 3. 37 ℃ thermostat Operation notes 1. Store the kit at 2-8 ° C and equilibrate at room temperature for 20 minutes before use. The concentrated washing liquid taken out of the refrigerator will have crystals, which is a normal phenomenon. The water bath is heated to completely dissolve the crystals before use. 2. The slats not used in the experiment should be immediately returned to the ziplock bag, sealed (dry at low temperature) and stored. 3. The standard dilution can be regarded as a negative control or blank; the sample after pretreatment does not need to be diluted, just take 10μL and add it. 4. Perform the incubation operation strictly in accordance with the time, amount of liquid and sequence indicated in the manual. 5. Shake all liquid components thoroughly before use. Kit composition name 96-well configuration 48 hole configuration Remarks Microplate 12 holes × 8 12 holes × 4 strips no Standard product (160pg / mL) 0.6mL 0.6mL Dilute according to the instructions Standard dilution 6mL 3mL no Sample diluent 6mL 3mL no Detection antibody-HRP 10mL 5mL no 20 × washing buffer 25mL 15mL Dilute according to the instructions Substrate A 6mL 3mL no Substrate B 6mL 3mL no Stop solution 6mL 3mL no Sealing film 2 sheets 2 sheets no Instructions 1 serving 1 serving no Ziplock bag 1 1 no Note: The standard product is diluted with standard product diluent in order: 160, 80, 40, 20, 10, 5pg / ml Reagent preparation Dilution of 20 × washing buffer: 1:20 dilution of distilled water, that is, 1 part of 20 × washing buffer plus 19 parts of distilled water. Washing method 1. Manually wash the plate: throw away all the liquid in the hole, fill each hole with the washing liquid, leave the liquid in the hole after standing for 1 min, pat dry on the absorbent paper, and wash the plate 5 times in this way. 2. Automatic plate washing machine: Inject 350μL of washing liquid into each well, soak for 1min, and wash the plate 5 times. Steps 1. Take out the required slats from the aluminum foil bag after equilibrating at room temperature for 20 min. The remaining slats are sealed with a ziplock bag and put back at 4 ° C. 2. Set up standard wells and sample wells, add 50μL of standard products of different concentrations to the standard wells; 3. Add 10μL of the sample to be tested first, and then add 40μL of the sample diluent; 4. Then add 100 μL of detection antibody labeled with horseradish peroxidase (HRP) to each well of the standard and sample wells, seal the reaction well with a sealing plate, and incubate for 60 min in a 37 ° C water bath or incubator. 5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1min, shake off the washing solution, pat dry on the absorbent paper, and repeat washing the plate 5 times (you can also wash the plate with a washing machine) 6. Add 50 μL of substrate A and B to each well, and incubate at 37 ° C in the dark for 15 minutes. 7. Add 50μL of stop solution to each well, and measure the OD value of each well at 450nm wavelength within 15min. Result judgment Draw standard curve: In the Excel worksheet, the standard product concentration is used as the abscissa, and the corresponding OD value is used as the ordinate. The standard product linear regression curve is drawn, and the concentration value of each sample is calculated according to the curve equation. Kit performance 1. Accuracy: The correlation coefficient R between the linear regression of the standard product and the expected concentration is greater than or equal to 0.9900. 2. Sensitivity: The minimum detection concentration is less than 1.0pg / ml. 3. Specificity: Does not cross-react with other soluble structural analogs. 4. Repeatability: The coefficients of variation within and between panels are less than 15%. 5. Storage: 2-8 ℃, protected from light and moisture. 6. Validity: 6 months

Many women believe too much in the test results of the pregnancy test. Once a positive result is confirmed, they are confirmed to be normal and have not been confirmed by the doctor. As everyone knows, a positive pregnancy test is not necessarily a normal pregnancy, it may be abnormal pregnancy such as ectopic pregnancy or hydatidiform mole. 20% expectant mothers mistakenly think that positive pregnancy test is normal pregnancy Many people will purchase pregnancy test sticks on their own. Once they respond positively, they will confirm normal pregnancy and have not been confirmed by the doctor. Until 6 to 8 weeks after the abnormal vaginal bleeding, abdominal pain, rushed to the hospital for treatment. A positive pregnancy test may not be a normal pregnancy, but may be an abnormal pregnancy such as ectopic pregnancy or hydatidiform mole. In general, after 6 weeks of pregnancy, the embryo sac can be seen from the ultrasound and the heartbeat can be heard. If the ultrasound does not see the embryo but the pregnancy is detected, it is strongly questioned whether it is ectopic pregnancy. Further blood tests are needed to detect beta-HCG. Pregnancy index to determine. How to use the highest accuracy of pregnancy test? First of all, some issues must be clarified: Ovulation is around the 14th day of the menstrual cycle. Assuming that fertilization is successful at this time, it takes the most time for the fertilized eggs to produce HCG for 6 or 7 days, and the pregnancy test sticks are judged by measuring urine HCG. of. However, HCG really began to secrete a large amount of secretory cells. After the embryo implantation, the syncytiotrophoblasts begin to secrete and produce HCG. It takes at least 11 days to get pregnant. Therefore, the first six days, if you want to be more accurate, then wait 11 days later, then more accurate. You can guess it yourself. The result of pregnancy (positive) can be read after 1 minute (fastest 30 seconds), referring to the fact that if two lines are clearly displayed after 1 minute in the test, the results are pregnant (positive) and do not have to wait until After 3 minutes, it is interpreted. The result of non-pregnancy must be determined after 3 minutes because the HCG hormone level in urine is very low during early pregnancy, and weak positive results may appear during the test. The result of weak positive may not be shown after 1 minute. With the increase of reaction time, the two lines will not be displayed until 3 minutes later. At this time, the two lines should still be interpreted as the result of pregnancy (positive); if there is still only one control line after 3 minutes, it can be interpreted as not Pregnancy (negative) results. The result of the interpretation after 5 minutes is invalid! This is because the interpretation within 5 minutes is used as an experimental basis for the design of products. The result of interpretation after more than 5 minutes or even longer is unclear. Some people may have false positive results. This result does not represent the actual situation.

This kit can only be used for scientific research, not for medical diagnosis Instruction manual of human anti-HCG antibody (HCG-Ab) ELISA detection kit Detection principle The kit uses double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with the capture antibody of human anti-HCG antibody (HCG-Ab), add the specimen, standard, and HRP-labeled detection antibody in sequence, incubate and wash thoroughly. The color is developed with the substrate TMB, which is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The color depth is positively correlated with the human anti-HCG antibody (HCG-Ab) in the sample. Measure the absorbance (OD value) at 450nm with a microplate reader to calculate the sample concentration. Sample collection, processing and storage methods 1. Serum: Use a test tube that does not contain pyrogens and endotoxins. Avoid any cell stimulation during the operation. After collecting blood, centrifuge at 3000 rpm for 10 minutes to quickly and carefully separate serum and red blood cells. 2. Plasma: EDTA, citrate or heparin anticoagulation. Take the supernatant by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: centrifuge at 3000 rpm for 10 minutes to remove particles and polymers. 4. Tissue homogenate: Add tissue to the right amount of saline and mash. Take the supernatant by centrifugation at 3000 rpm for 10 minutes. 5. Preservation: If the sample is not tested in time after collection, please aliquot it in one dose and freeze it at -20 ℃ to avoid repeated freezing and thawing. Thaw at room temperature and ensure that the sample is thawed evenly and fully. Bring your own items 1. Microplate reader (450nm) 2. High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL 3. 37 ℃ thermostat Operation notes 1. Store the kit at 2-8 ° C and equilibrate at room temperature for 20 minutes before use. The concentrated washing liquid taken out of the refrigerator will have crystals, which is a normal phenomenon. The water bath is heated to completely dissolve the crystals before use. 2. The slats not used in the experiment should be immediately returned to the ziplock bag, sealed (dry at low temperature) and stored. 3. The standard dilution can be regarded as a negative control or blank; the sample after pretreatment does not need to be diluted, just take 10μL and add it. 4. Perform the incubation operation strictly in accordance with the time, amount of liquid and sequence indicated in the manual. 5. Shake all liquid components thoroughly before use. Kit composition name 96-well configuration 48 hole configuration Remarks Microplate 12 holes × 8 12 holes × 4 strips no Standard product (160pg / mL) 0.6mL 0.6mL Dilute according to the instructions Standard dilution 6mL 3mL no Sample diluent 6mL 3mL no Detection antibody-HRP 10mL 5mL no 20 × washing buffer 25mL 15mL Dilute according to the instructions Substrate A 6mL 3mL no Substrate B 6mL 3mL no Stop solution 6mL 3mL no Sealing film 2 sheets 2 sheets no Instructions 1 serving 1 serving no Ziplock bag 1 1 no Note: The standard product is diluted with standard product diluent in order: 160, 80, 40, 20, 10, 5pg / ml Reagent preparation Dilution of 20 × washing buffer: 1:20 dilution of distilled water, that is, 1 part of 20 × washing buffer plus 19 parts of distilled water. Washing method 1. Manually wash the plate: throw away all the liquid in the hole, fill each hole with the washing liquid, leave the liquid in the hole after standing for 1 min, pat dry on the absorbent paper, and wash the plate 5 times in this way. 2. Automatic plate washing machine: Inject 350μL of washing liquid into each well, soak for 1min, and wash the plate 5 times. Steps 1. Take out the required slats from the aluminum foil bag after equilibrating at room temperature for 20 min. The remaining slats are sealed with a ziplock bag and put back at 4 ° C. 2. Set up standard wells and sample wells, add 50μL of standard products of different concentrations to the standard wells; 3. Add 10μL of the sample to be tested first, and then add 40μL of the sample diluent; 4. Then add 100 μL of detection antibody labeled with horseradish peroxidase (HRP) to each well of the standard and sample wells, seal the reaction well with a sealing plate, and incubate for 60 min in a 37 ° C water bath or incubator. 5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1min, shake off the washing solution, pat dry on the absorbent paper, and repeat washing the plate 5 times (you can also wash the plate with a washing machine) 6. Add 50 μL of substrate A and B to each well, and incubate at 37 ° C in the dark for 15 minutes. 7. Add 50μL of stop solution to each well, and measure the OD value of each well at 450nm wavelength within 15min. Result judgment Draw standard curve: In the Excel worksheet, the standard product concentration is used as the abscissa, and the corresponding OD value is used as the ordinate. The standard product linear regression curve is drawn, and the concentration value of each sample is calculated according to the curve equation. Kit performance 1. Accuracy: The correlation coefficient R between the linear regression of the standard product and the expected concentration is greater than or equal to 0.9900. 2. Sensitivity: The minimum detection concentration is less than 1.0pg / ml. 3. Specificity: Does not cross-react with other soluble structural analogs. 4. Repeatability: The coefficients of variation within and between panels are less than 15%. 5. Storage: 2-8 ℃, protected from light and moisture. 6. Validity: 6 months

Many people are very anxious, want to know as soon as possible if they are pregnant, to buy a very sensitive early pregnancy test paper, often bluffing. So, what is the correct method of using early pregnancy test strips and early pregnancy test strips? Generally 10 days after ovulation test, ovulation test strips can be measured positive or strong when measured on early pregnancy test paper shows weak weak or weak yang, this time not too worried to go to the hospital to confirm, continue to test the early pregnancy test paper the next day, may appear the following Happening: 1. If the color of the early pregnancy test paper is significantly deepened, 18 days after ovulation, you can go to the hospital to confirm. Because the HCG level should be doubled every two days when you are pregnant, so if the normal pregnancy, the color of the early pregnancy test paper will definitely deepen. Generally about 40 days of pregnancy (26 days after ovulation), early pregnancy test strips can be measured to strong Yang. If you continue to test the paper may occur early pregnancy test paper color fade phenomenon, this time do not worry, you can dilute in the urine and then test the water, (the amount of water dilution can be from less to more) if you can continue to measure after dilution Strong Yang, you can rest assured. If the color is weakened after dilution with water, you need to go to the hospital. 2. If it is found that the color of the early pregnancy test paper is not obvious, or weakened, if it is a biochemical pregnancy, it will come to menstruation, but it is recommended to continue to test early pregnancy test paper, has been measured until the negative. 3. If the early pregnancy test paper is not obvious, or weakened, if it is ectopic pregnancy, there may be vaginal bleeding, this situation may be indistinguishable from biochemical pregnancy, you can continue to test early pregnancy test paper, if the early pregnancy test paper can not be negative, or need Go to the hospital to exclude ectopic pregnancy. (This method is only suitable for about 35 days of pregnancy, ie about 25 days after ovulation). Early pregnancy test strips can also be used for abortion, after abortion can be measured twice a week early pregnancy test strips to see the color weakening, the normal situation of early pregnancy test paper color should gradually weaken, if you find that early pregnancy test paper does not weaken or deepen, Need to go to the hospital as soon as possible to review.

Pregnancy is the most amazing process of women in life, 300 days and nights, the baby grew up a little bit, the heart of the love is gradually getting stronger. Pregnancy of the second color of the world's pregnant pregnancy pregnant sun shade large-scale activities officially launched! This year's event encourages the display of the new generation of pregnant mothers, whether you are a cool girl in the workplace, or a pretty girl, or you are still a newly-grown baby, you can enjoy your baby's love and expectation In a unique way to express it, I believe fans will attract and praise. Organizers particularly meticulously set the "best maternal love", "the coolest pregnant mother award", "the tide of the latest motherhood award", "the most pregnant mother award", "the most pregnant mother award" and "the most pregnant pregnancy Mom Award ", as the" Charm Pregnancy Award "is even more generous awarded to every pregnant mother, to encourage" I pregnant with my wonderful "spirit of participation! In addition to the host "pregnant color" cash red envelopes, as well as pregnant mothers are well-known store goods, such as "Kwong Hall" month meal, Taiwan imported BENNY baby gift box, October crystal ready bag , As well as Happy Mummy fetal language instrument and so on. Beautiful gift in sight, the heart of the good Mommy who quickly sign up! Dance thumbs, sun "pregnant time" it! Please pay attention to the relevant content, "Pregnancy of China."

Porcine β-human chorionic gonadotropin (β-HCG) enzyme-linked immunoassay kit instruction manual This kit is for research use only. Drug Name: Generic Name: Porcine Beta Human Chorionic Gonadotropin (β-HCG) ELISA Kit purpose of usage: This kit is used for the determination of β-human chorionic gonadotropin (β-HCG) in pig serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of porcine β-human chorionic gonadotropin (β-HCG) in the specimen. Microporous plates were coated with purified porcine β-human chorionic gonadotropin (β-HCG) antibody to make solid-phase antibodies. Porcine β-human chorionic gonadotropin (β- HCG), then combined with HRP-labeled human villous gonadotropin β (β-HCG) antibody to form an antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with human villous gonadotropin β (β-HCG) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of porcine β-human chorionic gonadotropin (β-HCG) in the sample was calculated by a standard curve. Kit composition: 1 20 times concentrated washing solution 30ml × 1 bottle 7 stop solution 6ml × 1 bottle 2 Enzyme label reagent 6ml × 1 bottle 8 standard (270μg / L) 0.5ml × 1 bottle 3 Enzyme label coated plate 12 wells × 8 strips 9 standard dilutions 1.5ml × 1 bottle 4 Sample diluent 6ml × 1 bottle 10 instructions 1 copy 5 Developer A solution 6ml × 1 bottle 11 2 sealing film 6 Developer B liquid 6ml × 1 / bottle 12 sealed bag 1 Specimen requirements: 1. Specimens are extracted as soon as possible after collection, and extraction is performed according to relevant literature, and experiments should be conducted as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided 2. The samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity. Steps: 1. Dilution and loading of standard products: set 10 standard wells on the enzyme-coated plate, add 100 μl of the standard products in the first and second wells, and then add the standard products in the first and second wells. 50μl of diluent, mix well; then take 100μl from the first well and the second well and add them to the third and fourth wells respectively, and then add 50μl of standard dilution solution to the third and fourth wells respectively, mix well Then take 50μl each in the third and fourth wells and discard it, then add 50μl each to the fifth and sixth wells, and then add 50ul of the standard dilution solution to the fifth and sixth wells respectively, and mix well; After mixing, take 50μl from the fifth and sixth wells and add them to the seventh and eighth wells respectively. Then add 50μl of standard dilution solution to the seventh and eighth wells respectively. Take 50μl from the eight wells and add them to the ninth and tenth wells. Then add 50μl of the standard dilution solution to the ninth and tenth wells respectively. After mixing, take 50μl from the ninth and tenth wells and discard. (After dilution, the volume of each well is 50μl, and the concentrations are 180μg / L, 120μg / L, 60μg / L, 30μg / L, 15μg / L). 2. Add samples: set up blank wells (the blank control wells do not add samples and enzyme reagents, the rest of the steps are the same) and the sample wells to be tested. Add 40μl of sample diluent to the test sample well of the enzyme-coated plate, and then add 10μl of the test sample (the final dilution of the sample is 5 times). Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix. 3. Incubation: seal the plate with the sealing film and incubate at 37 ° C for 30 minutes. 4. Mixing solution: dilute 20 times concentrated washing liquid with distilled water 20 times and reserve 5. Washing: Carefully peel off the sealing film, discard the liquid, spin dry, fill each well with the washing solution, let it stand for 30 seconds and discard, repeat 5 times and pat dry. 6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well. 7. Incubation: The operation is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: add 50μl of developer A to each well, and then add 50μl of developer B, mix gently, and develop color at 37 ℃ in the dark 15 minutes. 10. Termination: Add 50μl of stop solution to each well to stop the reaction (at this time the blue will turn to yellow). 11. Determination: Measure the absorbance (OD value) of each well in sequence with the blank air conditioner at zero and 450 nm wavelength. The measurement should be carried out within 15 minutes after adding the stop solution. Calculation: Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or use the concentration of the standard Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply it by the dilution factor to obtain the actual concentration of the sample. Precautions: 1. The kit should be taken out of the refrigerated environment and equilibrated at room temperature for 15-30 minutes before use. If the enzyme-coated plate is unsealed after opening, the strip should be stored in a sealed bag. 2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing. 3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples. 4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the substance to be tested in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with a certain multiple (n times) of the sample diluent and then determine it. Multiple (× n × 5). 5. The sealing film is limited to one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined by the microplate reader. 8. All samples, washing liquids and various wastes should be treated as infectious agents. 9. The components of different batches of this reagent shall not be mixed. 10. If there is any difference with the English manual, the English manual shall prevail. examination range: 10μg / L -200μg / L specification: 96 servings / box storage conditions and expiration date 1. Kit storage :; 2-8 ℃. 2. Validity: 6 months

Human anti-HCG antibody IgA (HCG-IgA) ELISA Kit Instructions for Use This reagent is for research use only Purpose: This kit is used to determine the content of anti-HCG antibody IgA (HCG-IgA) in human serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human anti-HCG antibody IgA (HCG-IgA) in the specimen. Microporous plates were coated with purified human anti-HCG antibody IgA (HCG-IgA) antibody to prepare solid-phase antibodies. Anti-HCG antibody IgA (HCG-IgA) was added to the microwells coated with mAb in turn, and then labeled with HRP The goat anti-human antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the anti-HCG antibody IgA (HCG-IgA) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human anti-HCG antibody IgA (HCG-IgA) in the sample was calculated by a standard curve. Kit composition: Kit composition 48 well configuration 96 well configuration storage instructions 1 part 1 part sealing plate film 2 pieces (48) 2 pieces (96) One sealed bag and one enzyme-labeled coating plate 1 × 48 1 × 96 2-8 ℃ Preserved standard: 54pg / ml 0.5ml × 1 bottle 0.5ml × 1 Preserved standard diluent 1.5ml × 2-8 ℃ × 1 bottle of 1.5ml × 1 bottle of enzyme-labeled reagents stored at 2-8 ℃ 3 ml × 1 bottle of 6 ml × 1 bottles of sample diluent at 2-8 ℃ 3 ml × 1 bottle of 6 ml × 1 bottles of color storage at 2-8 ℃ Reagent A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C storage developer B solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C storage stop solution 3ml × 1 bottle 6ml × 1 bottle 2- Store the concentrated washing solution at 8 ℃ (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle at 2-8 ℃ 1. Serum: room temperature blood coagulates naturally for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. 2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen, mixed for 10-20 minutes, and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 3. Urine: collected in sterile tubes and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, centrifuge again. Pleural and ascites, cerebrospinal fluid reference implementation. 4. Cell culture supernatant: When detecting secreted components, collect with a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When detecting the components inside the cells, dilute the cell suspension with PBS (PH7.2-7.4), and the cell concentration will reach about 1 million / ml. Through repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 5. Organize the specimen: after cutting the specimen, weigh it. Add a certain amount of PBS, PH7.4. Quickly freeze and save with liquid nitrogen for later use. After the specimen melts, it still maintains a temperature of 2-8 ° C. Add a certain amount of PBS (PH7.4) and homogenize the specimen with a manual or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. After aliquoting, a portion is to be tested, and the rest is frozen for future use. 6. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided. 7. The sample containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP). Steps: 1. Dilution and loading of standard products: set 10 standard wells on the enzyme-coated plate, add 100 μl of the standard products in the first and second wells, and then add the standard products in the first and second wells. 50μl of diluent, mix well; then take 100μl from the first well and the second well and add them to the third and fourth wells respectively, and then add 50μl of standard dilution solution to the third and fourth wells respectively, mix well Then take 50μl each in the third and fourth wells and discard it, then add 50μl each to the fifth and sixth wells, and then add 50ul of the standard dilution solution to the fifth and sixth wells respectively, and mix well; After mixing, take 50μl from the fifth and sixth wells and add them to the seventh and eighth wells respectively. Then add 50μl of standard dilution solution to the seventh and eighth wells respectively. Take 50μl from the eight wells and add them to the ninth and tenth wells. Then add 50μl of the standard dilution solution to the ninth and tenth wells respectively. After mixing, take 50μl from the ninth and tenth wells and discard. (After dilution, the volume of each well is 50μl, and the concentrations are 36pg / ml, 24pg / ml, 12pg / ml, 6pg / ml, 3pg / ml). 2. Add samples: set up blank wells (the blank control wells do not add samples and enzyme reagents, the rest of the steps are the same) and the sample wells to be tested. Add 40μl of sample diluent to the test sample well of the enzyme-coated plate, and then add 10μl of the test sample (the final dilution of the sample is 5 times). Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix. 3. Incubation: seal the plate with the sealing film and incubate at 37 ° C for 30 minutes. 4. Mixing solution: Dilute 30 times (20 times of 48T) concentrated washing liquid with distilled water 30 times (20 times of 48T) and then use. 5. Washing: Carefully peel off the sealing film, discard the liquid, spin dry, fill each well with the washing solution, let it stand for 30 seconds and discard, repeat 5 times and pat dry. 6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well. 7. Incubation: The operation is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: add 50μl of developer A to each well, and then add 50μl of developer B, mix gently, and display at 37 ℃ for 15 minutes in the dark. 10. Termination: Add 50μl of stop solution to each well to stop the reaction (at this time the blue will turn to yellow). 11. Determination: Measure the absorbance (OD value) of each well in sequence with the blank air conditioner at zero and 450 nm wavelength. The measurement should be performed within 15 minutes after adding the final solution. Precautions: 1. The kit should be equilibrated at room temperature for 15-30 minutes before being taken out of the refrigerated environment. If the enzyme label coated plate is unopened, the strip should be stored in a sealed bag. 2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing. 3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples. 4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the substance to be tested in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with a certain multiple (n times) of the sample diluent and then determine it. Multiple (× n × 5). 5. The sealing film is limited to one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined by the microplate reader. 8. All samples, washing liquids and various wastes should be treated as infectious agents. 9. The components of different batches of this reagent shall not be mixed. 10. If there is any difference with the English manual, the English manual shall prevail. Calculation: Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; then multiply by the dilution factor; Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply it by the dilution factor to obtain the actual concentration of the sample. (This picture is for reference only) Kit performance: 1. The correlation coefficient R between the linear regression of the sample and the expected concentration is more than 0.990. 2. The batch and approval shall be less than 9% and 11% respectively examination range: 1pg / ml -45pg / ml Storage conditions and validity period: 1. Store the kit: 2-8 ℃. 2. Validity: 6 months

Human anti-HCG antibody IgA (HCG-IgA) ELISA Kit Instructions for Use This reagent is for research use only Purpose: This kit is used to determine the content of anti-HCG antibody IgA (HCG-IgA) in human serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human anti-HCG antibody IgA (HCG-IgA) in the specimen. Microporous plates were coated with purified human anti-HCG antibody IgA (HCG-IgA) antibody to prepare solid-phase antibodies. Anti-HCG antibody IgA (HCG-IgA) was added to the microwells coated with mAb in turn, and then labeled with HRP The goat anti-human antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the anti-HCG antibody IgA (HCG-IgA) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human anti-HCG antibody IgA (HCG-IgA) in the sample was calculated by a standard curve. Kit composition: Kit composition 48 well configuration 96 well configuration storage instructions 1 part 1 part sealing plate film 2 pieces (48) 2 pieces (96) One sealed bag and one enzyme-labeled coating plate 1 × 48 1 × 96 2-8 ℃ Preserved standard: 54pg / ml 0.5ml × 1 bottle 0.5ml × 1 Preserved standard diluent 1.5ml × 2-8 ℃ × 1 bottle of 1.5ml × 1 bottle of enzyme-labeled reagents stored at 2-8 ℃ 3 ml × 1 bottle of 6 ml × 1 bottles of sample diluent at 2-8 ℃ 3 ml × 1 bottle of 6 ml × 1 bottles of color storage at 2-8 ℃ Reagent A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C storage developer B solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C storage stop solution 3ml × 1 bottle 6ml × 1 bottle 2- Store the concentrated washing solution at 8 ℃ (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle at 2-8 ℃ 1. Serum: room temperature blood coagulates naturally for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. 2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen, mixed for 10-20 minutes, and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 3. Urine: collected in sterile tubes and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, centrifuge again. Pleural and ascites, cerebrospinal fluid reference implementation. 4. Cell culture supernatant: When detecting secreted components, collect with a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When detecting the components inside the cells, dilute the cell suspension with PBS (PH7.2-7.4), and the cell concentration will reach about 1 million / ml. Through repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 5. Organize the specimen: after cutting the specimen, weigh it. Add a certain amount of PBS, PH7.4. Quickly freeze and save with liquid nitrogen for later use. After the specimen melts, it still maintains a temperature of 2-8 ° C. Add a certain amount of PBS (PH7.4) and homogenize the specimen with a manual or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. After aliquoting, a portion is to be tested, and the rest is frozen for future use. 6. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided. 7. The sample containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP). Steps: 1. Dilution and loading of standard products: set 10 standard wells on the enzyme-coated plate, add 100 μl of the standard products in the first and second wells, and then add the standard products in the first and second wells. 50μl of diluent, mix well; then take 100μl from the first well and the second well and add them to the third and fourth wells respectively, and then add 50μl of standard dilution solution to the third and fourth wells respectively, mix well Then take 50μl each in the third and fourth wells and discard it, then add 50μl each to the fifth and sixth wells, and then add 50ul of the standard dilution solution to the fifth and sixth wells respectively, and mix well; After mixing, take 50μl from the fifth and sixth wells and add them to the seventh and eighth wells respectively. Then add 50μl of standard dilution solution to the seventh and eighth wells respectively. Take 50μl from the eight wells and add them to the ninth and tenth wells. Then add 50μl of the standard dilution solution to the ninth and tenth wells respectively. After mixing, take 50μl from the ninth and tenth wells and discard. (After dilution, the volume of each well is 50μl, and the concentrations are 36pg / ml, 24pg / ml, 12pg / ml, 6pg / ml, 3pg / ml). 2. Add samples: set up blank wells (the blank control wells do not add samples and enzyme reagents, the rest of the steps are the same) and the sample wells to be tested. Add 40μl of sample diluent to the test sample well of the enzyme-coated plate, and then add 10μl of the test sample (the final dilution of the sample is 5 times). Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix. 3. Incubation: seal the plate with the sealing film and incubate at 37 ° C for 30 minutes. 4. Mixing solution: Dilute 30 times (20 times of 48T) concentrated washing liquid with distilled water 30 times (20 times of 48T) and then use. 5. Washing: Carefully peel off the sealing film, discard the liquid, spin dry, fill each well with the washing solution, let it stand for 30 seconds and discard, repeat 5 times and pat dry. 6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well. 7. Incubation: The operation is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: add 50μl of developer A to each well, and then add 50μl of developer B, mix gently, and display at 37 ℃ for 15 minutes in the dark. 10. Termination: Add 50μl of stop solution to each well to stop the reaction (at this time the blue will turn to yellow). 11. Determination: Measure the absorbance (OD value) of each well in sequence with the blank air conditioner at zero and 450 nm wavelength. The measurement should be performed within 15 minutes after adding the final solution. Precautions: 1. The kit should be equilibrated at room temperature for 15-30 minutes before being taken out of the refrigerated environment. If the enzyme label coated plate is unopened, the strip should be stored in a sealed bag. 2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing. 3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples. 4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the substance to be tested in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with a certain multiple (n times) of the sample diluent and then determine it. Multiple (× n × 5). 5. The sealing film is limited to one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined by the microplate reader. 8. All samples, washing liquids and various wastes should be treated as infectious agents. 9. The components of different batches of this reagent shall not be mixed. 10. If there is any difference with the English manual, the English manual shall prevail. Calculation: Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; then multiply by the dilution factor; Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply it by the dilution factor to obtain the actual concentration of the sample. (This picture is for reference only) Kit performance: 1. The correlation coefficient R between the linear regression of the sample and the expected concentration is more than 0.990. 2. The batch and approval shall be less than 9% and 11% respectively examination range: 1pg / ml -45pg / ml Storage conditions and validity period: 1. Store the kit: 2-8 ℃. 2. Validity: 6 months

According to the physiological and psychological characteristics of women during pregnancy, the company's professional design team has developed a series of pregnant women's fashion and underwear that are suitable for all seasons to make up for the beauty of women during pregnancy, with new fabrics, fashionable styles, and sophisticated craftsmanship and professional production Play my own shortcomings.

1. Influence of variety According to data, the average number of ovulation in Chinese pig breeds was 17.21 at the beginning of production and 21.58 at production. For example, there were 25.68 Chinese Jiaxing black pigs, 28.0 Erhualian and 15 Neijiang pigs. . The number of pigs born in foreign pigs was 13.5, and 21.4 pigs were produced. For example, the average White pigs were 16.7, Duroc 11.5, and Landrace 15.22. The number of ovulation was different. 2, hybridization can increase the number of ovulation can increase the number of sows ovulation, has been verified by many tests, is a relatively easy to implement method. In the organization of production, most of the local breed sows that have a higher number of litters are female parents, and the male pigs that have introduced pig breeds are male parents. After selection, the sow hybrid females continue to be female parents, and then introduce one more import pig. The male boar is the second male parent, and all the offspring are fattened. As a result of this, full use of the heterozygous generation has the characteristics of better ovulation, which can increase production efficiency. The [Sanyo" hybrid implemented in the market is to use this feature to formulate a production model. 3. The number of ovulations of different parity sows differs from that of primiparous and sows. The number of ovulations also changes with the increase of parity in the sows. Generally speaking, the number of litters will gradually increase during the period of 2-6 months, and after 6 months, especially after 8 months, The number of ovulation shows a decreasing trend. This law of change provides a basis for us to reasonably adjust the parity of sows. That is, the age of the sow group should not be too small or too large, and it should be ensured that the proportion of sows with a majority of 3-5 births is a good proportion. 4. The number of ovulations in pigs is affected by temperature. According to Mr. Xu Shiqing's measurement, the number of ovulation in Jiaxing black pigs is 6.5 °C in winter, and the number of ovulation is 23.3, while the ovulation number is 34.4 in summer temperature at 21-24 °C. That is, a suitable high temperature can promote multiple ovulation of sows. However, too high a temperature can affect the ability of the sow to ovulate normally. In the production, it is necessary to adjust the temperature in the house with the change of climate. It is important to promote the normal estrus of sows and ovulation. 5, Nutritional supply data on sows ovulation data reported that the level of nutrition has little effect on the number of ovulation in pigs. Such as high nutrient levels, the number of ovulation was 13.9 and the low nutrient level was 13.6. However, there are also reports that when gilts are in heat, short-term superior feeding can increase the number of ovulation by 1-2 pieces, which has a certain use value in production. 6, the application of sex hormones can affect the number of ovulation Practice shows that sows after sexual maturity, or after weaning appropriate selection of female hormones, such as pregnant horse serum, estradiol, etc., can adjust the endocrine changes in sows, promote sows Timely heat, and the possibility of abnormal ovulation, makes it possible to increase the number of sows born. 7, gilts aphrodisiac supplementation can increase the number of ovulation gilts appear estrus signs began to increase the daily dietary supply, increase the amount of 40-50% of the original amount, you can make sows increase the number of ovulation more than 2 . This method does not apply to adult sows. 8. The number of ovulation of sows after weaning to short-term pre-breeding (feeding) can be increased by one.

"I am a Special Forces 3 fire Phoenix" is a story about the special warfare women's forces. The 21st century is a century in which talented people and high-tech dominate the war. They come from different positions in different units and hold together their own ideals. Almost brutal training and combat them gradually transformed, and ultimately to a real military attitude to bloom again, the Phoenix Nirvana. Pregnancy of the color go hand in hand with your handsome uniform wind dress, the traditional military uniform - mighty, handsome, rigid, Russian uniforms sexy, attractive, rigid and flexible side of the female reflected most vividly! Selling points: Soldiers in the winter popular patch cloth embroidered take outside the military green big hat coat with slim pencil pants, the whole with a slim body, play cool winter, how can the winter wardrobe less their existence yet. Selling point: elegant little collard dress, fungus flouncing skirt, outside take cocoon thicker sweater. Neutral color, whether intellectual or you are elegant, can control.

Byron Geldard, a 19-year-old man from the United Kingdom, suffered from physical discomfort last year. After he had finished the ultrasound in the hospital, the doctor initially diagnosed that he had a tumor in his body and had spread to the lungs, but he was not sure which type of cancer he had. He was later referred to the Cambridge hospital by the Teenage Cancer Trust and was asked to have a urine checkup. After the doctor used the pregnancy test, the results were positive. Geldard was diagnosed with stage 4 cancer, and the cancer cells spread to the abdominal cavity and lungs, and chemotherapy was immediately performed. Fortunately, after repeated chemotherapy, his hormone index gradually decreased from the super high standard, and the tumor began to gradually decrease. He was treated with tumor resection in December last year and has recovered. Experts pointed out that in recent years, the use of pregnancy test rods to detect testicular cancer is becoming more and more common. The main reason is that the testicular cancer produces chorionic gonadotropin hormone (hCG Hormone), which is also produced in female pregnancy when the placenta grows.

Correct use of early pregnancy test strips: To improve the accuracy of early pregnancy test strips, we must grasp the correct use of early pregnancy test strips: 1. Read the instruction manual thoroughly before performing the test. Return the reagent and urine sample to room temperature (20°C to 30°C) before use. 2. Remove the reagent strip from the original packaging foil bag and use it within 1 hour as soon as possible. 3. Insert the reagent strip into the urine specimen in the direction of the arrow. Note: The urine level must not exceed the marking line of the reagent strip. 4. Take at least 5 seconds and take out the observations on a clean flat surface. 5. Wait for the appearance of the magenta band. The test result should be read at 3 minutes. After 10 minutes, the judgement is invalid. How to determine the results of early pregnancy test paper? 1. Positive (+): Two magenta bands appear. One is in the test area (T) and the other is in the quality control area (C). Indicates that she is pregnant. 2. Negative (-): Only a magenta band appears in the quality control zone (C), and no magenta band appears in the test zone (T). Indicates that she is not pregnant. 3. Invalid: The magenta band does not appear in the quality control area (C), indicating that the process was incorrect or that the reagent strip has been deteriorating. Note: The color bands of the reagent strip control line and the test line may show color depth due to the amount of hCG in the urine, and the results are judged to be in accordance with the above criteria. What should you watch out for when using a pregnancy test strip? 1. Early pregnancy test papers purchased by you that are stored for too long (more than one year), or early pregnancy test papers are damp, and they are not stored under normal room temperature conditions (not cold-cold), they may fail, and false positives result. . 2. If the pregnancy has just started, or if there is a possibility of ectopic pregnancy (ectopic pregnancy), the samples with low HCG levels in the body need to stand for more than 3 minutes (usually only 1 minute), and you must carefully identify whether there is a weak positive - - The detection zone only appears faintly. 3. What should I pay attention to when using pregnancy test strips? After 3 months of pregnancy, HCG levels decline, and urine tests sometimes appear negative or weakly positive. 4. In extreme cases, such as hydatidiform mole, choriocarcinoma, HCG levels in the body will be too high, urine testing does not show positive. 5, positive results do not mean 100% pregnancy. Because some tumor cells such as hydatidiform mole, choriocarcinoma, bronchial carcinoma and kidney cancer, also can secrete HCG. We also had positive HCG tests in patients with endometrial hyperplasia.